Epidemiological and Molecular study for Malta Fever
DOI:
https://doi.org/10.59658/jkas.v4i5.687Keywords:
Brucellosis, Rose Bengal test, Culture, PCR.Abstract
The objective of study was diagnosis of some Brucella spp. from human suspects and infection patients via methods of serology (Rose Bengal test) and culture. The PCR assay was investigated as a potential role in detection of some Brucella. Blood samples was used to detection common species like: Brucella melitensis and Brucella abortus from sick persons whom were suspected to be infected with brucellosis.
Blood and serum tests were gotten from individuals whom were suspected of contamination with brucellosis, alluded to numerous doctor's facilities in various city of Baghdad (Karkh and Rusafa parts), which include: (General doctor's facility Mohammad Baqir Al-Hakim, Al-Shaheed Al-Sadder clinic, Al-Imam Ali (rest in peace) doctor's facility), and access to insights and maps in all Iraq areas from Ministry of Health/Communicable Disease Control Center, within the time period of the research that lasted from (March to December of the year 2014) .
A sum of 117 fringe blood tests was acquired from sick persons about whom there were suspects of contamination with brucellosis. The analysis of brucellosis was affirmed by clinical discoveries by utilizing exams of serological nature such as: Rose Bengal test, culture and Gram recoloring and distinctive biochemical exams. To build up a PCR system for conclusion of brucellosis, DNA extraction was done through utilizing a business pack, followed by PCR amplification by using two sets of primers: B4/B5 and IS711 B.melitensis and B.abortus.
The results were obtained as 70 (59.82%) specimens as affirmative outcome by RBT and 59 (50.42%) specimens were affirmative outcome by culture from the blood of sick persons. Once the PCR method had been used to blood specimens as, 30 (25.64) cases positive for Brucella species, and 20 (17.09 %) were positive for Brucella melitensis and Brucella abortus.
These outcomes demonstrate that sick persons were in contact with creature of domesticated animals that contaminated or associated disease with Brucella like: sheep, goat, cow and bison situated in epidemiological locales in Iraq ,particularly in Baghdad region over the research time frame and indicated that blood culture technique is vital for the discovery of brucellosis in contrast with serological strategies (Rose Bengal test) for the analysis of brucellosis. The PCR strategy is prescribed as an other option to culture for the analysis of brucellosis, so PCR method gives quick finding of brucellosis, which is important to begin the process of curing of a particular sick person.
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2017 Journal of Kerbala for Agricultural Sciences
This work is licensed under a Creative Commons Attribution 4.0 International License.
Licensing Terms
All articles are published under a Creative Commons License and will be directed to the Creative Commons Attribution 4.0 International License That permits use, distribution, and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.
Use by non-commercial users
For non-commercial and non-promotional purposes individual users may access, download, copy, display, and redistribute the articles to colleagues, as well as adapt, translate, text- and data-mine the content subject to the following conditions:
- The author's moral rights are not compromised. These rights include the right of "paternity" (also known as "attribution" - the right for the author to be identified as such) and "integrity" (the right for the author not to have the work altered in such a way that the author's reputation, or integrity may be impugned).
- Where content in the article is identified as belonging to a third party, it is the obligation of the user to ensure that any reuse complies with the copyright policies of the owner of that content.
- If article content is copied, downloaded, or otherwise reused for non-commercial research and education purposes, a link to the appropriate bibliographic citation (authors, journal, article title, volume, issue, page numbers, DOI, and the link to the definitive published version on JKAS website) should be maintained.
- Copyright notices and disclaimers must not be deleted.
- Any translations, for which a prior translation agreement with JKAS has not been agreed, must prominently display the statement: "This is an unofficial translation of an article that appeared in an FSP publication. The publisher has not endorsed this translation."
Use by commercial "for-profit" organizations
Use of JKAS Open Access articles for commercial, promotional, or marketing purposes requires further explicit permission from JKAS (journal.agri@uokerbala.edu.iq) and will be subject to a fee.
The commercial purposes include:
Copying or downloading of articles, or linking to such articles for further redistribution, sale, or licensing; Copying, downloading, or posting by a site or service that incorporates advertising with such content; The inclusion, or incorporation of article content in other works, or services (other than normal quotations with an appropriate citation) that is then available for sale or licensing, for a fee (for example, a compilation produced for marketing purposes, inclusion in a sales pack); Use of article content (other than normal quotations with appropriate citation) by for-profit organizations for promotional purposes; Linking to article content in e-mails redistributed for promotional, marketing or educational purposes; Use for the purposes of monetary reward by means of sale, resale, license, loan, transfer or other form of commercial exploitation such as marketing products; Print reprints of articles can be purchased from journal.agri@uokerbala.edu.iq.
Permissions
- No special permission is required to reuse all, or part of the article published by JKAS, including figures and tables for non-commercial purposes.
- Any part of the article may be reused, for non-commercial purposes, without permission provided that the original article is cited.
- Reuse of an article does not imply endorsement by the authors, JKAS
