Effect of adding probiotic on experimental in vitro digestion and rumen fluid characteristics of cows
DOI:
https://doi.org/10.59658/jkas.v13i1.5743Keywords:
probiotic, Bacillus subtilis,Bacillus licheniformi, digestion experiment, number of anaerobic bacteria.Abstract
The aim of this study was to demonstrate effect use of probiotic that contains many of bacteria such as Clostridium butyricum, Bacillus subtilis, Bacillus licheniformis estimate the digestion coefficient of dry and organic matter. The concentration of ammonia nitrogen, use of 3 treatments, to measurement of percentage of dry matter ,organic matter, the amount of metabolized energy, the percentage of protein, and the total numbers of anaerobic bacteria in the rumen fluid. The results showed that there were no significant differences between all treatments in the character the percentage of organic matter digestibility (OM%) and the percentage of dry matter and organic matter(DM), and no differed significantly from each other in the organic matter digestibility coefficient (IVOMD). When the result for (IVDMD) recorded high differed between treatment so each treat of probiotic (T2 and T3) superiority on treat control (T1). also the results of the statistical analysis to the (NH3N) showed a highly significant superiority of the two probiotic treatments over the control treatment, as the averages reached 0.48, 0.69, and 0.81 for both the control treatment and the probiotic at concentrations of 0.02 and 0.04, respectively. The results of the statistical analysis of the protein percentage showed a highly significant value of p<0.01 The probiotic treatment with a concentration of 0.04 and 0.02 was significantly different from the control treatment the control treatment and the probiotic for both concentrations reached 2.97, 4.30 and 5.07%, respectively. The statistical analysis indicated that there was no significant effect of using a probiotic in estimating the amount of metabolized energy for each treatment, The results of the statistical analysis for the number of microorganisms showed that the two coefficients were superior. The probiotic treatment was significantly superior to the control treatment, as the numbers of bacteria reached 72.33, 196.66, and 228.33 Bacterial colony units/ml of rumen fluid for both the control and probiotic treatments at concentrations of 0.02 and 0.04, respectively.
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2026 Copyright (c) 2024 is the Author's article. Published by the Journal of Kerbala for Agricultural Sciences under a CC BY 4.0 license
This work is licensed under a Creative Commons Attribution 4.0 International License.
Licensing Terms
All articles are published under a Creative Commons License and will be directed to the Creative Commons Attribution 4.0 International License (CC BY 4.0) That permits use, distribution, and reproduction in any medium, provided the original work is properly cited. This license also allows the work to be used for commercial purposes.
Use by both non-commercial and commercial users
This content is licensed under a Creative Commons Attribution 4.0 International (CC BY 4.0) license, permitting use by both non-commercial and commercial users. Individual users may access, download, copy, display, and redistribute the articles to colleagues, as well as adapt, translate, and text- and data-mine the content, subject to the following conditions:
- The author's moral rights, including the right of attribution and the right to protect their work from derogatory treatment, are respected.
- Where content in the article is identified as belonging to a third party, users must ensure that any reuse complies with the copyright policies of the owner of that content.
- If the article content is reused for research or educational purposes, users should maintain a link to the appropriate bibliographic citation, including the DOI and a link to the published version on the journal's website.
